2019, Volume 11, Issue 3
TNFA expression level changes observed in response to the Wingate Anaerobic Test in non-trained and trained individuals
Agnieszka Maciejewska-Skrendo1, Jan Mieszkowski2, Andrzej Kochanowicz1, Błażej Stankiewicz2, Paweł Cięszczyk1, Katarzyna Świtała1, Gilmara Gomes de Assis3, Katarzyna Kecler4, Maciej Tarnowski4, Marek Sawczuk1
1Gdansk University of Physical Education and Sport in Gdansk
2Kazimierz Wielki University in Bydgoszcz
3Mossakowski Medical Research Centre, Polish Academy of Science
4Pomeranian Medical University in Szczecin
Author for correspondence: Agnieszka Maciejewska-Skrendo; Gdansk University of Physical Education and Sport in Gdansk; email: firstname.lastname@example.org
Background: Literature reports indicate changes in the expression of genes encoding proinflammatory factors are observed as a result of physical exercise. The stress changes caused by high-intensity loads and adaptive changes induced by the planned long-term training are less studied. The aim of this study was to determine the impact of intense anaerobic effort in people adapted to regular trainings and in non-trained persons on the expression levels of the TNFA gene.
Material and methods: 50 experienced soccer players and 50 non-trained participants were recruited for the study. Anaerobic capacity was measured by means of the Wingate Anaerobic Test. To evaluate the expression of TNFA gene, a QRT-PCR was applied.
Results: A comparison of the TNFA expression profiles between well-trained athletes and non-trained controls revealed that transcript levels were higher in non-trained participants when compared with soccer players in all the studied time points, with the exception of the second post-test; however, this difference was the only one that was statistically insignificant.
Conclusions: The TNFA mRNA expression characteristic described in our study indicated a significant downregulation of the TNFA expression observed in the course of time in experienced athletes reflecting molecular adaptation to physical effort caused by long-term training regime.
Key words: TNFA, TNFα, tumor necrosis factor alpha, gene expression changes, Wingate Anaerobic Test